The present invention relates to a reverse osmosis membrane including a porous support including a polysulfone layer; and a polyamide active layer formed on the porous support, wherein a concentration of sulfur (S) detected on a surface of the polyamide active layer is 1.8×10−3 units or less on average or a concentration of sulfur dioxide (SO2) detected on the surface of the polyamide active layer is 7×10−3 units or less on average, and a method of manufacturing a reverse osmosis membrane, the method including forming a porous support; and forming a polyamide active layer on the porous support by interfacially polymerizing a polyfunctional amine solution including a carbodiimide compound, and a polyfunctional acid halide compound solution.