Invention Grant
US09133516B2 Methods for identification of alleles using allele-specific primers for amplification
有权
使用等位基因特异性引物鉴定等位基因进行扩增的方法
- Patent Title: Methods for identification of alleles using allele-specific primers for amplification
- Patent Title (中): 使用等位基因特异性引物鉴定等位基因进行扩增的方法
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Application No.: US12305193Application Date: 2007-06-29
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Publication No.: US09133516B2Publication Date: 2015-09-15
- Inventor: Caixia Li , Huafang Gao , Xiang Liu , Bin Cai , Di Zhang , Jing Cheng
- Applicant: Caixia Li , Huafang Gao , Xiang Liu , Bin Cai , Di Zhang , Jing Cheng
- Applicant Address: CN Beijing CN Beijing
- Assignee: CapitalBio Corporation,Tsinghua University
- Current Assignee: CapitalBio Corporation,Tsinghua University
- Current Assignee Address: CN Beijing CN Beijing
- Agency: Morrison & Foerster LLP
- Priority: CN200610089526 20060630
- International Application: PCT/CN2007/002039 WO 20070629
- International Announcement: WO2008/003244 WO 20080110
- Main IPC: C12Q1/68
- IPC: C12Q1/68

Abstract:
The invention provides a method for identification of alleles. In this method, genomic DNA is used as target. Multiple allele-specific PCR amplification are carried out with a group of primers comprising one or more allele-specific primers for a target gene, a universal primer, and a common primer; and a DNA polymerase without 5′ to 3′ exonuclease activity. The PCR products are hybridized with tag probes immobilized on a DNA chip. Results are determined based on the signal intensity and the position of the probe immobilized on the array. Each allele-specific primer comprises a unique tag sequence at the 5′ end. Each tag probe immobilized on the DNA chip comprises a sequence identical to its corresponding tag sequence; and each tag probe hybridizes only with the complementary sequence in the PCR amplification product.
Public/Granted literature
- US20100041563A1 METHODS FOR IDENTIFICATION OF ALLELES Public/Granted day:2010-02-18
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