An electronic device and a method of setting control information using the same are provided. The method includes displaying an application, which includes a plurality of media items that are reproducible according to individually adjustable control information; identifying one of the plurality of media items for which associated adjustable control information is to be adjusted responsive to a first user input; displaying a control region including at least one user-definable control parameter individually defining the adjustable control information associated with the identified one of the plurality of media items; and adjusting the adjustable control information associated with the identified one of the plurality of media items responsive to a second user input.