An interface circuit for signal transmission includes an amplifying circuit, a de-skew circuit and a latching unit. The amplifying circuit receives an input clock signal and outputs an output clock signal after amplifying the input clock signal. The de-skew circuit receives the output clock signal and outputs a de-skew clock signal as a trigger signal after removing a skew time of the output clock signal. The latching unit includes multiple sampling circuits, respectively receives multiple inputting data signals. The sampling circuits are controlled by the trigger signal to sample the inputting data signals and output multiple outputting data signals. The voltage amplitudes of the outputting data signals are larger than the voltage amplitudes of the inputting data signals and satisfy a required voltage amplitude by a subsequent circuit.