A fluorescence imaging module includes an image sensor and a lens disposed between a fluorescence sample and the image sensor to focus a fluorescence image of the fluorescence sample onto the image sensor. The fluorescence sample is to be positioned an object distance away from the lens. The lens is positioned an image distance away from the image sensor. The image distance is greater than the object distance. An illuminating device is disposed between the fluorescence sample and the lens. The illuminating device includes a light source and an optical element. The light source is adapted to emit light in a first direction towards the optical element. The optical element is optically coupled to receive the light and redirect the light in a second direction towards the fluorescence sample to illuminate the fluorescence sample.