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US08377676B2 Mutated nucleotide sequences of batroxobin, mutated α factor secretion signal sequence and processes for preparing batroxobin using the same 有权
巴曲酶的突变核苷酸序列，突变的α因子分泌信号序列和使用其制备巴曲酶的方法

Mutated nucleotide sequences of batroxobin, mutated α factor secretion signal sequence and processes for preparing batroxobin using the same
Abstract:
The present invention relates to a batroxobin-encoding nucleotide sequence and/or a mutated α-factor secretion signal sequence, and a vector and a transformant using the same. The batroxobin-encoding nucleotide sequence of this invention exhibits an excellent expression efficiency in yeast, particular Pichia pastoris and the recombinant batroxobin is obtained at 4-13 fold higher yield than natural-occurring batroxobin-encoding sequences. The protein expression system which uses the batroxobin-encoding nucleotide sequence as well as mutated α-factor secretion signal peptide sequence of this invention obtains the recombinant batroxobin at about 20-fold higher yield than natural-occurring batroxobin-encoding sequences. In addition, the recombinant batroxobin prepared using the sequence of this invention has a significantly plausible activity and stability compared with natural-occurring batroxobin.
Abstract(Chinese):

本发明涉及一种编码巴曲酶的核苷酸序列和/或突变的α因子分泌信号序列，以及使用其的载体和转化体。 本发明的巴曲酶编码核苷酸序列在酵母，特别是巴斯德毕赤酵母中表现出优异的表达效率，并且以比天然存在的巴曲酶编码序列高出4-13倍的产量获得重组巴曲酶。 使用巴曲酶编码核苷酸序列以及本发明的突变型α因子分泌信号肽序列的蛋白质表达系统以比天然存在的巴曲酶编码序列高出约20倍的产量获得重组巴曲酶。 此外，使用本发明的序列制备的重组巴曲酶与天然存在的巴曲酶相比具有显着似乎合理的活性和稳定性。

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