There are provided an interlayer film separation solution and an interlayer film separation method capable of separating an interlayer film and a glass for a short period of time and collecting the separated interlayer film in a recyclable condition. An interlayer film separation solution 21 having etching ability for a glass is introduced into a container 22, and the introduced interlayer film separation solution 21 was controlled to a temperature of 30 to 60° C., and a laminated glass 10 with glass plates 11, 12 crushed is introduced into a barrel 23 from a lid portion 23a, and simultaneously, 50 to 60 metal pieces 25 are, introduced into the barrel 23 from the lid portion 23a in order to easily separate the laminated glass 10 into the glass plates 11, 12 and an interlayer film 13. The barrel 23 containing the laminated glass 10 and the metal pieces 25 introduced thereinto is rotated at a predetermined rotational speed.