Invention Grant
US07524646B2 Method for diversifying the chemical composition of proteins produced in vivo by genetically disabling the editing function of their aminoacyl tRNA synthetases
失效
通过遗传上禁用其氨基tRNA合成酶的编辑功能使体内产生的蛋白质的化学成分多样化的方法
- Patent Title: Method for diversifying the chemical composition of proteins produced in vivo by genetically disabling the editing function of their aminoacyl tRNA synthetases
- Patent Title (中): 通过遗传上禁用其氨基tRNA合成酶的编辑功能使体内产生的蛋白质的化学成分多样化的方法
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Application No.: US11054192Application Date: 2005-02-08
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Publication No.: US07524646B2Publication Date: 2009-04-28
- Inventor: Volker Doring , Leslie A. Nangle , Tamara L. Hendrickson , Valerie De Crecy-Lagard , Paul Schimmel , Philippe Marliere
- Applicant: Volker Doring , Leslie A. Nangle , Tamara L. Hendrickson , Valerie De Crecy-Lagard , Paul Schimmel , Philippe Marliere
- Applicant Address: FR Paris US CA La Jolla DE Cologne
- Assignee: Institut Pasteur,The Scripps Research Institute,Evologic GmbH
- Current Assignee: Institut Pasteur,The Scripps Research Institute,Evologic GmbH
- Current Assignee Address: FR Paris US CA La Jolla DE Cologne
- Agency: Bozicevic, Field & Francis, LLP
- Agent Michael B. Rubin
- Main IPC: C12P21/06
- IPC: C12P21/06 ; C12N9/10 ; C12N9/12
Abstract:
The present invention is directed to a method to diversify the chemical composition of proteins produced in vivo comprising the step of disabling, particularly by mutagenesis, the editing function of one of its aminoacyl tRNA synthetases. The present invention is also directed to nucleic acid sequences encoding such mutated aminoacyl tRNA synthetases having their editing site mutated and capable of mischarging its cognate tRNA with a noncanonical amino acid. Also described herein is an improved method for obtaining transformed cells capable of synthetizing in vivo proteins comprising at least a noncanonical amino acid and their use for the production of such proteins.
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