An antibacterial composition is provided that has an anionic surfactant, an amphoteric surfactant, a cationic conditioning agent, an antibacterial agent and water. Preferably, the composition is a liquid hand soap. The aqueous antibacterial composition, especially when an antibacterial liquid hand soap, has a proper balance of ingredients for providing a high cleansing ability, high foam, good conditioning and a pleasant emollient effect on the skin. The method of reducing amount of bacteria on part of a person's body, such as a hand, includes contacting the hand with the composition and, thereafter, rinsing the hand with water.