发明授权
US5753220A Cysteine protease gene defective baculovirus, process for its production, and process for the production of economic protein by using the same 失效
半胱氨酸蛋白酶基因缺陷型杆状病毒,其生产过程,以及通过使用其生产经济蛋白质的方法

Cysteine protease gene defective baculovirus, process for its
production, and process for the production of economic protein by using
the same
摘要:
For the expression of a foreign gene, a virus is provided in which at least a part of a cysteine protease gene present on a genome of a baculovirus has been deleted or substituted by a marker gene. A process is also disclosed for the formation of the virus. A transfer vector for the deletion of a cysteine protease gene is also described. This transfer vector comprises a DNA fragment of a nuclear polyhedrosis virus genome, in which at least a part of a cysteine protease gene has been substituted by a marker gene, and a plasmid vector fragment. Also disclosed is a process for the production of an economic protein. This production process comprises inoculating a host insect with a gene recombination virus, said gene recombination virus having been obtained by deleting or substituting by a marker gene at least a part of a cysteine protease gene present on a genome of a baculovirus or a nuclear polyhedrosis virus; expressing an economic protein gene inserted downstream a polyhedral gene promoter, granulin gene promoter or p10 gene promoter of the gene recombination virus; and collecting the economic protein.
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