发明授权
US5753220A Cysteine protease gene defective baculovirus, process for its
production, and process for the production of economic protein by using
the same
失效
半胱氨酸蛋白酶基因缺陷型杆状病毒,其生产过程,以及通过使用其生产经济蛋白质的方法
- 专利标题: Cysteine protease gene defective baculovirus, process for its production, and process for the production of economic protein by using the same
- 专利标题(中): 半胱氨酸蛋白酶基因缺陷型杆状病毒,其生产过程,以及通过使用其生产经济蛋白质的方法
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申请号: US404445申请日: 1995-03-15
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公开(公告)号: US5753220A公开(公告)日: 1998-05-19
- 发明人: Takeo Suzuki , Akihiro Usami , Kouhei Oda , Hajime Mori , Toshimichi Kanaya
- 申请人: Takeo Suzuki , Akihiro Usami , Kouhei Oda , Hajime Mori , Toshimichi Kanaya
- 申请人地址: JPX Tokyo
- 专利权人: Katakura Industries Co., Ltd.
- 当前专利权人: Katakura Industries Co., Ltd.
- 当前专利权人地址: JPX Tokyo
- 优先权: JPX6-068961 19940315; JPX7-023296 19950119
- 主分类号: A01K67/033
- IPC分类号: A01K67/033 ; A61K48/00 ; C07K14/61 ; C12N7/00 ; C12N15/09 ; C12N15/866 ; C12P21/00 ; C12N15/64 ; C12N15/86
摘要:
For the expression of a foreign gene, a virus is provided in which at least a part of a cysteine protease gene present on a genome of a baculovirus has been deleted or substituted by a marker gene. A process is also disclosed for the formation of the virus. A transfer vector for the deletion of a cysteine protease gene is also described. This transfer vector comprises a DNA fragment of a nuclear polyhedrosis virus genome, in which at least a part of a cysteine protease gene has been substituted by a marker gene, and a plasmid vector fragment. Also disclosed is a process for the production of an economic protein. This production process comprises inoculating a host insect with a gene recombination virus, said gene recombination virus having been obtained by deleting or substituting by a marker gene at least a part of a cysteine protease gene present on a genome of a baculovirus or a nuclear polyhedrosis virus; expressing an economic protein gene inserted downstream a polyhedral gene promoter, granulin gene promoter or p10 gene promoter of the gene recombination virus; and collecting the economic protein.
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