A collection and separation device for use in combination with a fluid collection container operatively coupled to a suction source to withdraw body fluids and collect tissue specimens from a patient comprising a tissue collection container operatively coupled between the patient and the fluid collection container having at least one tissue collection basket removably disposed therein to receive body fluids and tissue specimens from the patient including a separator to separate the body fluids and tissue specimens and a fluid collection chamber or zone disposed adjacent the separator such that body fluids and tissue specimens from the patient are received in the tissue collection basket where the body fluids and tissue specimens are separated whereby the tissue specimen is retained in the tissue collection basket for subsequent removal from the tissue collection container and the body fluids are collected in the fluid collection chamber or zone for collection and removal to the fluid collection container.