The invention provides a display device and a Mura compensation method thereof. The display device includes a display panel and a Mura compensation circuit. The Mura compensation circuit defines a compensation area in the display panel based on a curve equation corresponding to a current frame rate of the display panel. The Mura compensation circuit calculates a compensation weight in the compensation area based on the current frame rate and the curve equation. The Mura compensation circuit uses the compensation weight to compensate a pixel data in the compensation area so as to generate a compensated pixel data frame. The Mura compensation circuit provides the compensated pixel data frame to the display panel for displaying images.