Provided is an HLA gene amplification primer, comprising primers of any one or more groups of the following nine primer groups: a first primer group: SEQ ID NO: 1 and SEQ ID NO: 2; a second primer group: SEQ ID NO: 3 and SEQ ID NO: 4; a third primer group: SEQ ID NO: 5 and SEQ ID NO: 6; a fourth primer group: SEQ ID NO: 7 and SEQ ID NO: 8; a fifth primer group: SEQ ID NO: 9 and SEQ ID NO: 10; a sixth primer group: SEQ ID NO: 11 and SEQ ID NO: 12; a seventh primer group: SEQ ID NO: 12 and SEQ ID NO: 13; an eighth primer group: SEQ ID NO: 14 and SEQ ID NO: 15; and a ninth primer group: SEQ ID NO: 16, SEQ ID NO: 17, and SEQ ID NO: 18. Also provided is a kit for HLA gene sequencing, which comprises the described HLA gene amplification primer; provided is a gene sequencing library construction method based on the described HLA gene amplification primer and an HLA gene sequencing method based on the HLA gene sequencing library construction method.