Provided are a display substrate and a display apparatus. A display substrate includes a display area and a bonding area, the display area includes a first display area and a second display area; the first display area includes a first sub-pixel and a first data line, and the second display area includes a second sub-pixel and a second data line; the bonding area includes a detection circuit, the detection circuit includes a control line, a first signal line, a second signal line, a first detection sub-unit and a second detection sub-unit; the first detection sub-unit is configured to output a signal transmitted by the first signal line to the first data line, and the second detection sub-unit is configured to output a signal transmitted by the second signal line to the second data line.