An electronic device with plugging and unplugging function is provided. The electronic device has a connector. The connector is adapted to be connected to a connection port of an electronic module. The electronic device includes a tray unit, a housing and a moving mechanism. The tray unit is moveably disposed in the housing. The moving mechanism is disposed on the housing, wherein the tray unit moves the moving mechanism, and the moving mechanism moves the connector to be connected to or disconnected from the connection port.