A display substrate, including: a base substrate; a pixel defining layer including: a plurality of first blocking portions spaced in a second direction, a plurality of second blocking portions spaced in a first direction and a plurality of barriers spaced in the first direction, each first blocking portion is parallel to the first direction, each second blocking portion is parallel to the second direction, the plurality of first blocking portions and second blocking portions surround to form a plurality of pixel light-emitting regions, and at least one barrier is disposed between adjacent second blocking portions in the second direction; a plurality of accommodating regions, at least one thereof is located between adjacent first blocking portions in the second direction and between adjacent second blocking portion and barrier in the first direction; and a first diversion structure disposed on a side of the second blocking portion away from the base substrate.