Disclosed is a display substrate including a display region and a non-display region. The non-display region is provided with a gate drive circuit, the gate drive circuit includes a plurality of cascaded shift register units, and a shift register unit is connected with at least one power supply line. The shift register unit includes a first output circuit and a second output circuit. The first output circuit is connected with a first group of clock signal lines, and the second output circuit is connected with the first group of clock signal lines and a second group of clock signal lines. In a first direction, the first group of clock signal lines and the at least one power supply line are located between the first output circuit and the second output circuit.