A bicycle wheel comprises a rim and a valve assembly. The rim includes a radially inner wall having an inner opening and a radially outer wall having an outer opening. A first portion of the rim includes a first securing feature (e.g., inner threads). The valve assembly is positioned in the inner opening and outer opening and includes a second securing feature (e.g., outer threads) engaging the first securing feature. The rim can include a plate that defines the first engaging feature. The valve assembly preferably includes a valve stem and a resilient bumper coupled to the valve stem and aligned with a second portion of the rim. The valve assembly can further include a seal member compressed between the valve stem and the rim. Preferably, the seal member is positioned in an annular groove in an underside of an enlarged head of the valve stem.