Invention Publication
- Patent Title: Method for Cell Line Development
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Application No.: US18467658Application Date: 2023-09-14
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Publication No.: US20240043879A1Publication Date: 2024-02-08
- Inventor: Daniel Ivansson , Andreas Castan
- Applicant: Cytiva Sweden AB
- Applicant Address: SE Uppsala
- Assignee: Cytiva Sweden AB
- Current Assignee: Cytiva Sweden AB
- Current Assignee Address: SE Uppsala
- Priority: GB 03418.2 2017.03.03
- The original application number of the division: US16490623 2019.09.03
- Main IPC: C12N15/90
- IPC: C12N15/90 ; C12P21/00 ; C07K16/00
Abstract:
The present invention relates to an improved method for cell line development (CLD) which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells.
The method combines site-directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI) and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts using SDI from that point on.
The method combines site-directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI) and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts using SDI from that point on.
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