Provided is a liquid cell for an electron microscope according to an embodiment of the inventive concept, including a lower widow layer, an upper widow layer above the lower widow layer, and a separation film disposed between the upper widow layer and the lower widow layer so as to separate the upper widow layer and the lower widow layer from each other. An upper space may be defined by the upper widow layer and the separation film, and a lower space may be defined by the lower widow layer and the separation film. The upper widow layer, the lower widow layer, and the separation film may include the same material.