A display substrate has a display region and a peripheral region. The display substrate includes a substrate, a first dam, a second dam and a connection portion. The first dam and the second dam are located on a side of the substrate, and are located in the peripheral region. The second dam is farther from the display region than the first dam. A height of the second dam is greater than a height of the first dam. The connection portion is located between the first dam and the second dam. The connection portion connects the first dam and the second dam, and a height of the connection portion is less than the height of the first dam. At least a portion of the first dam, at least a portion of the connection portion and at least a portion of the second dam are of an integrative structure.