A method and an apparatus of uplink transmission and reception in a wireless communication system is disclosed. A method of transmitting uplink control information by a terminal in a wireless communication system according to an embodiment of the present disclosure may include receiving, from a base station, a plurality of physical downlink shared channels (PDSCHs); and transmitting, to the base station, the uplink control information including Hybrid Automatic Repeat and reQuest-Acknowledgment (HARQ-ACK) information based on the plurality of PDSCHs. Here, the plurality of PDSCHs include a plurality of semi-persistent scheduling (SPS) PDSCHs, and based on HARQ-ACK information for the plurality of SPS PDSCHs being multiplexed by appending to pre-configured HARQ-ACK information, the HARQ-ACK information included in the uplink control information is determined by excluding at least one SPS PDSCH associated with a disabled HARQ process among the plurality of SPS PDSCHs.