A surgical instrument for applying surgical clips to tissue comprises an end effector having first and second jaws that are movable between open and closed positions and configured to receive first and second rows of clips in the open position. The instrument further includes an actuator coupled to the end effector and configured to move the jaws into the closed position and to discharge the first and second rows of clips. This allows an operator to apply multiple rows of clips to tissue with a single instrument insertion, which obviates the need to remove the surgical instrument from the cannula to manually reload a new cartridge, thereby reducing the overall time of the surgical procedure.