Aspects of the disclosure relate to using synthetic DNA stranding and mutant nucleotide processes to conduct enterprise market volatility predictions. In some embodiments, a computing platform may initiate a set of instructions associated with performing an action on a synthetic DNA market data set associated with a plurality of lines of business across an enterprise organization. Thereafter, the computing platform may convert the set of instructions to a mutant nucleotide sequence, and insert the mutant nucleotide sequence into the synthetic DNA market data set. The computing platform may extract, using the mutant nucleotide sequence, target information from the synthetic DNA market data set, and validate the target information to detect one or more anomalies. The computing platform may remove the one or more data anomalies, and subsequently output a validated synthetic DNA market data set to a synthetic DNA client server.