The present invention discloses methods and a device for performing cardiology and endovascular interventional operations via a radial artery or ulnar artery. The method includes: step 1, putting an artery sheath into a punctured artery, wherein the artery is a radial artery or ulnar artery; step 2, allowing a guiding catheter to penetrate into the artery sheath from outside the body and penetrate out of the artery sheath from the other end to be advanced to a left ventricle; step 3, advancing a myocardial biopsy forcep to the left ventricle endocardium through the guiding catheter to collect a piece of myocardium; step 4, withdrawing the myocardial biopsy forcep from the guiding catheter to be outside the body, and preserving the myocardium specimen; step 5, repeating the step 3 and the step 4 until collecting enough number of specimens; and step 6, withdrawing the guiding catheter from the artery sheath, pulling the artery sheath out of the artery, and performing compression dressing over the access site. When performing myocardial biopsy and other relevant operations, the method does not need to penetrate other blood vessels or pull the artery sheath out of the artery and exchange the artery sheath with other sheathless guiding catheter or catheter, thereby making the procedure more convenient and more effective, as well as reducing complications caused by stimuli to the puncture site.