A timing controller and an anti-interference method thereof are provided. The timing controller includes a timing control circuit. The timing control circuit provides an input signal for controlling a source driver. When at least one of the timing control circuit and the source driver detects that an interference event occurs to the input signal, the timing control circuit is configured to adjust a frequency of a data signal or a clock signal from a normal operation frequency to at least one anti-interference frequency. The timing control signal is further configured to provide at least one of the data signal and the clock signal to the source driver.