An antimicrobial nonwoven wet wipe includes i) a fibrous nonwoven substrate bonded with a cross-linkable VAE dispersion stabilized with one or more nonionic colloidal stabilizers and one or more nonionic surfactants, and ii) absorbed in the nonwoven substrate, an aqueous lotion including one or more cationic disinfectants. No anionic surfactants are present in the antimicrobial nonwoven wet wipe. A method of producing the antimicrobial nonwoven wet wipe includes a) applying a first aqueous composition including a crosslinkable VAE dispersion stabilized with one or more nonionic colloidal stabilizers and one or more nonionic surfactants to a nonwoven substrate; b) drying the composition; and c) applying a second aqueous composition to the product of step b). At least one of the first and second aqueous compositions includes one or more cationic disinfectants.