Systems and methods for non-invasive brain stimulation using focused ultrasound are provided. An exemplary method of non-invasive brain stimulation in the brain of a subject can include securing the subject in a fixed position relative to an ultrasound source and providing a focused ultrasound having one or more ultrasound parameters to a location in the brain of the subject, the location and the one or more ultrasound parameters selected to evoke a physical response of the subject, and measuring the physical response of the subject.