Methods and apparatuses for time alignment calibration are provided including acquiring an event-stream and video images of a target object which are simultaneously shot by a dynamic vision sensor and an assistant vision sensor, determining a key frame that reflects obvious movement of the target object from the video images, mapping effective pixel positions of the target object in the key frame and effective pixel positions of the target object in the neighboring frames according to a spatial relative relation between the dynamic vision sensor and the assistant vision sensor, determining a first target object template that covers events in a first event-stream segment from the plurality of target object templates, and using a time alignment relation of an intermediate instant of the first event-stream segment and a timestamp of a frame corresponding to the first target object template between the dynamic vision sensor and the assistant vision sensor.