A method includes pressing a macromolecular material to form a successive reflective layer, heating the reflective layer to a preset temperature value, stirring luminous powder and fluorescent powder at a high speed so that the luminous powder and the fluorescent powder are mixed evenly to form a luminous blank, evenly painting the luminous blank on the reflective layer, drying the luminous blank at a predetermined temperature value to form a luminous layer, and cooling the reflective layer and the luminous layer to produce a substrate which has reflective and self-luminous features. Thus, the luminous layer of the substrate emits light in a dark environment by reflection of the reflective layer of the substrate.