The present disclosure relates to a 5G or pre-5G communication system that will be provided to support a higher data transmission rate beyond a 4G communication system such as LTE. Disclosed is an interference measurement method and device in a flexible duplex system. The method comprises the steps of: determining whether uplink (UL) grant for allocating UL transmission has been received from a base station in a first subframe; when the UL grant has not been received, measuring inter-cell interference for an uplink interference measurement resource (IMR) in at least one second subframe determined by the first subframe; and when the UL grant has been received, measuring inter-cell interference for the uplink interference measurement resource (IMR) in at least one third subframe before a subframe that is indicated by the UL grant.