It is disclosed a scrubbing and sampling device, a card reader apparatus and a gate apparatus. The scrubbing and sampling device includes: a scrubbing and sampling portion including a first wheel and a second wheel, which are respectively capable of rotating around respective rotating axes, and a scrubbing conveyor belt tensioned by the first wheel and the second wheel and driven by rotation of the first wheel and the second wheel, to move between them; and an desorbing portion configured to desorb properties of an sample that is conveyed into the desorbing portion. The scrubbing conveyor belt is configured to move through the desorbing portion such that the desorbing portion desorbs the sample on the scrubbing conveyor belt when the scrubbing conveyor belt enters the desorbing portion.