A precision positioning device of a unit-type die cutting & hot stamping machine includes a positioning element on an input end of a first imprinting element and a positioning element on an output end of each imprinting element. Two neighboring positioning elements accomplish the positioning between the two positioning elements together. Each positioning element is constituted by a positioning assembly installed symmetrically on each end of the imprinting element. The positioning assembly includes a positioning rod which can rotate against an installation shaft. Each positioning rod includes a positioning block which is provided with a reference surface. A rear side of the gripper is provided with a reference matching surface. By the positioning elements, the accuracy in the art of die cutting & hot stamping can be assured when the machine is performing a multiple-working-position procedure.