A manufacturing method of a flexible substrate is provided. The method comprises: coating a flexible substrate material and drying it; using a mask to expose the dried flexible substrate material, wherein the mask has a plurality of opaque regions distanced from each other, and the opaque regions form a designed pattern; developing the flexible substrate material to remove a portion of the flexible substrate material except the opaque regions; and curing the flexible substrate material to form a plurality of films comprising the flexible substrate material, which correspond to the designed pattern.