发明申请
- 专利标题： Labeled Biomolecular Compositions and Methods for the Production and Uses Thereof
- 专利标题（中）： 标记的生物分子组合物及其生产和使用方法
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申请号： US13902228申请日： 2013-05-24
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公开(公告)号： US20140011228A1公开(公告)日： 2014-01-09
- 发明人: Gaetano T. Montelione , Masayori Inoue , Yuefeng Tang , Mouica Roth , William Schneider
- 申请人： Gaetano T. Montelione , Masayori Inoue , Yuefeng Tang , Mouica Roth , William Schneider
- 申请人地址： US NJ Somerset
- 专利权人： University of Medicine and Dentistry of New Jersey
- 当前专利权人： University of Medicine and Dentistry of New Jersey
- 当前专利权人地址： US NJ Somerset
- 主分类号： C12N15/63
- IPC分类号： C12N15/63 ; G01N24/08
摘要：
Disclosed herein is a set of E. coli single-protein production (SPP) technologies with protein NMR (SPP-NMR) for (i) using isotope-enriched membrane proteins produced with the SPP system in screening detergent conditions suitable for purification and/or three-dimensional structure analysis without the requirement for protein purification, (ii) producing 2H, 13C, 15N enriched proteins suitable for high throughput and membrane protein NMR studies, and (iii) labeling with 13C—15N specific peptide bonds in proteins (referred to herein as SPP-PBL).
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