Methods and systems to determine and regulate the gain of gamma-ray detectors in tools equipped with neutron sources are provided. Gain regulation may be based on the gamma ray background generated in the tool by interaction of neutrons with the nuclei of some elements constituting the tool structure. Some chemical elements that are used in construction of the tool's mechanical parts and structure result in characteristic gamma ray lines in a measured energy spectrum. These lines can be used to calibrate the energy scale of the response of the detector, without any added calibration radio isotopic source.