The present invention relates to a container for the collection of urine over a period of 24 hours, the inner volume of which is defined by a bottom wall, a perimetral wall and a top wall. The container comprises at least a first access mouth made on the top wall or perimetral wall, and means for drawing off the urine collected in the container. These means comprise a cup body inserted inside the container to close the first access mouth. The cup body delimits a chamber for housing a vacuum vial. On the bottom of the cup body there is an aperture which places the chamber in communication with the inner volume. These drawing means further comprise means for fluidically coupling the aperture to the vacuum vial, and a tube for sucking the urine. This tube is fluidically coupled to the aperture and extending from the bottom of the cup body as far as the bottom wall of the container.