A displacement syringe having a housing with a distal tip and a proximal end; a moveable outer plunger within the housing having an elongated passageway; at least one moveable inner plunger within the passageway of the outer plunger; at least one first seal between the outer plunger and the housing; and at least one second seal between the inner plunger and the outer plunger is disclosed herein. The syringe may have a lock at the proximal end of the housing and/or a valve at the distal tip of the housing. The syringe may also have a plurality of inner plungers of various diameters. A transfer system and delivery system are also disclosed herein. Methods to accurately and efficiently deliver small volumes using the displacement syringe are also disclosed.