Disclosed is a separation/detection column that can detect a component separated by a separating agent having optical responsivity which is the same as that of a component in a sample. The separation/detection column is configured by filling one end of a tube 1, having ultraviolet transparency, with a first filler that has ultraviolet responsivity and a separation ability to separate the component in the sample, and by filling the other end of the tube 1 with a second filler that has ultraviolet responsivity which is different from that of the first filler and does not have the separation ability via a spacer 4 constituted by quartz wool, and moreover by inserting an end cap 5 constituted by quartz wool into the other end of the tube 1.