A tire tread rubber composition includes: diene rubber containing three kinds of rubber (E-SBR, modified S-SBR, and natural rubber (NR)) in a total amount of 80% by weight or more; and a filler in an amount of 100 to 140 parts by weight based on 100 parts by weight of the diene rubber, the filler containing 70% by weight or more of silica, wherein the diene rubber contains 10 to 25% by weight of the natural rubber, the weight ratio of the three kinds of rubber (E-SBR:modified S-SBR:NR) is 1 to 2:2.5 to 4:1, the styrene content of the E-SBR and the modified S-SBR is 35 to 40% by weight, and the difference between the glass transition temperature of the E-SBR and the glass transition temperature of the modified S-SBR is 10° C. or lower.