An auto-zero amplifier is disclosed, having an amplifying circuit, a switch, and a difference signal generating circuit. The amplifying circuit receives a first input signal for generating a first output signal, and receives a second input signal for generating a second output signal. The switch is coupled between the amplifying circuit and a capacitor. The switch is conducted for charging or discharging the capacitor to a voltage with the first output signal, and the switch is not conducted for keeping the capacitor at the voltage. The difference signal generating circuit is coupled with the amplifying circuit and the capacitor for generating a difference signal of the first output signal and the second output signal, a multiple of the difference signal, a part of the difference signal, and/or a digital output value for the difference signal.