The present invention relates to an automatic golf ball supply device for automatically supplying golf balls to a position where the user hits the golf ball using a golf club. A characterising feature of the present invention is that it comprises: a frame; an ascending and descending vertical carrier provided in such a way that it is free to ascend and descend; a motor; a first rod for performing a forwards rotation or backwards rotation movement; a second rod linked in movement to the other end of the first rod; a separating type of pressed-member which separates from the ascending and descending vertical carrier when the ascending and descending vertical carrier is ascending, and which collides with and is pressed by a pushing plate formed in the other side surface of the ascending and descending vertical carrier when the ascending and descending vertical carrier is descending; a rotational force transmission means for converting the pressing force of the pressed-member into a rotational force; and a golf ball guide member means for nudging the golf ball and settling it on a tee member of the ascending and descending vertical carrier when the vertical carrier has descended, by means of the rotational force transmission means. As a result, the present invention is advantageous in that it reduces production costs, lessens the incidence of malfunction, and can maintain a flexible performance in operation in diverse environments.