Methods and systems that can launch applications into existing isolation environments do so by executing a run module on a computing machine to intercept requests to execute an application. A client communicating with the computing machine generates requests to execute an application on the computing machine. A run module identifies a profile associated with the requested application and queries an application delivery service to identify at least one isolation environment that corresponds to the profile. The run module receives from the application delivery service a response that identifies a first isolation environment associated with the application, and issues a command to a launch module to launch the application into the first isolation environment.