Provided is a biochip including a high-sensitivity image sensor. The biochip includes: a biochip layer including a plurality of reaction zones in which biochemical reactions occur formed as concaves, the reaction zone including a reference material at a lower portion and a target material at an upper portion; and an image sensor layer which is formed below the biochip layer and includes a plurality of photo detectors. Since the biochip is implemented as a single chip including the biochip layer and the image sensor layer, light loss in the luminescence or fluorescence operation can be reduced. In addition, additional devices such as a scanner which are needed for a general biochip are not needed, so that sensitivity is improved, and low-cost biochips can be implemented.