Provision of a multilayer structure superior in the alcohol gasoline permeation-preventing property, interlayer adhesiveness, low temperature impact resistance, heat resistance and chemical resistance. A multilayer structure comprising at least two layers of layer A consisting of a polyamide resin composition (a) comprising 30-90 mass % of a polyamide resin (X) comprising a dicarboxylic acid unit comprising 50-100 mol % of a terephthalic acid unit and/or a lo naphthalene dicarboxylic acid unit, and a diamine unit comprising 60-100 mol % of an aliphatic diamine unit having 9-13 carbon atoms, and 70-10 mass % of an impact resistance modifier, and layer B consisting of a polyamide resin composition (b) comprising 50-95 mass % of a polyamide resin (X′) comprising a dicarboxylic acid unit comprising 50-100 mol % of a terephthalic acid unit and/or a naphthalene dicarboxylic acid unit, and a diamine unit comprising 60-100 mol % of an aliphatic diamine unit having 9-13 carbon atoms, and 50-5 mass % of an impact resistance modifier, which satisfies Y≧Y′+5 wherein Y shows a content ratio (mass %) of the impact resistance modifier in layer A and Y′ shows a content ratio (mass %) of the impact resistance modifier in layer B.