发明申请
- 专利标题: System and method for smoothing melting curve data
- 专利标题(中): 熔化曲线数据的系统和方法
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申请号: US10852788申请日: 2004-05-25
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公开(公告)号: US20050255483A1公开(公告)日: 2005-11-17
- 发明人: Alan Orr , Roger Taylor
- 申请人: Alan Orr , Roger Taylor
- 专利权人: Stratagene California
- 当前专利权人: Stratagene California
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; G01N33/48 ; G01N33/50 ; G06F19/00
摘要:
The present invention is a system and method for smoothing melting curve data to help identify a specific sequence of double stranded DNA sample after performing a polymerase chain reaction. The system comprises a plurality of measurements of a fluorescence and a temperature of a dye over a temperature range to create a raw array; calculating a collapse number using a temperature resolution that facilitates the smoothing of the melting curve data, a number of data points in the raw array, and a temperature data range from the raw array; calculating a plurality of fluorescence averages and a plurality of temperature averages from the collapse number and the raw array; and generating a smooth array from the plurality of fluorescence averages and the plurality of temperature averages wherein the smooth array decreases a variability in the raw array.
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