Provided is a display substrate including a display region and a non-display region. The non-display region is provided with a gate drive circuit, and the gate drive circuit includes a plurality of cascaded shift register units; a shift register unit includes an input sub-circuit and a denoising output sub-circuit. The denoising output sub-circuit is connected with the input sub-circuit, a first group of clock signal lines, and a second group of clock signal lines, and the input sub-circuit is connected with a third group of clock signal lines. The third group of clock signal lines, the input sub-circuit, the first group of clock signal lines, the denoising output sub-circuit, and the second group of clock signal lines are sequentially arranged along a first direction.