- 专利标题: Method for producing galactooligosaccharides
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申请号: US17834149申请日: 2022-06-07
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公开(公告)号: US12043855B2公开(公告)日: 2024-07-23
- 发明人: Sven-Rainer Döring , Marco Steffens
- 申请人: DMK Deutsches Milchkontor GmbH
- 申请人地址: DE Zeven
- 专利权人: DMK Deutsches Milchkontor GmbH
- 当前专利权人: DMK Deutsches Milchkontor GmbH
- 当前专利权人地址: DE Zeven
- 代理机构: Dilworth & Barrese, LLP
- 优先权: EP 178340 2021.06.08
- 主分类号: C12P19/14
- IPC分类号: C12P19/14 ; A23C9/12 ; A23C21/02 ; C08B37/00 ; C12N9/38 ; C12P19/00
摘要:
Suggested is method for producing galactooligosaccharides, comprising the following steps:
(i) Providing an aqueous milk sugar solution;
(ii) Sterilising the milk sugar solution;
(iii) Performing a transgalactosilation of the milk sugars present in the sterilised milk sugar solution of step (ii) by adding at least one beta-galactosidase within its optimum temperature and pH value intervals for a period of at least 30 minutes, obtaining a reaction mixture;
(iv) Inhibiting the enzyme mass in the reaction mixture of step (iii), and
(v) Confectioning the reaction mixture of step (iv),
Wherein
(a) The enzyme mass is wholly or partly inhibited by setting a pH value outside the activity optimum of the enzymes;
(b) The reaction mixture, together with the inhibited enzyme mass, is subjected to filtration, obtaining a retentate (R1) and a permeate (P1);
(c) The inhibited amount of enzymes is separated as a retentate (R1) and is fed back to step (iii); and
(d) The permeate (P2) containing the galactooligosaccharides is passed on to step (v).
(i) Providing an aqueous milk sugar solution;
(ii) Sterilising the milk sugar solution;
(iii) Performing a transgalactosilation of the milk sugars present in the sterilised milk sugar solution of step (ii) by adding at least one beta-galactosidase within its optimum temperature and pH value intervals for a period of at least 30 minutes, obtaining a reaction mixture;
(iv) Inhibiting the enzyme mass in the reaction mixture of step (iii), and
(v) Confectioning the reaction mixture of step (iv),
Wherein
(a) The enzyme mass is wholly or partly inhibited by setting a pH value outside the activity optimum of the enzymes;
(b) The reaction mixture, together with the inhibited enzyme mass, is subjected to filtration, obtaining a retentate (R1) and a permeate (P1);
(c) The inhibited amount of enzymes is separated as a retentate (R1) and is fed back to step (iii); and
(d) The permeate (P2) containing the galactooligosaccharides is passed on to step (v).
公开/授权文献
- US20220411455A1 METHOD FOR PRODUCING GALACTOOLIGOSACCHARIDES 公开/授权日:2022-12-29
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