Disclosed herein are an adhesion blocking element, a three-dimensional printing device and a three-dimensional printing method. The adhesion blocking element comprises: one light-transmittable main body comprising a first surface and a second surface which are disposed opposite to each other, and side faces connecting the first surface and the second surface; and a plurality of microstructures arranged on the main body, wherein each microstructure has one cavity formed in the main body and one first open face which is arranged on the first surface of the main body and communicated to the cavity. The present invention decreases the adhesion between the adhesion blocking element and the cured layer by improving the structure of the adhesion blocking element itself, and eliminates the negative pressure adsorption between the cured layer and the adhesion blocking element, so that it is easier to peel the adhesion blocking element off from the cured layer. Meanwhile, the service life of the adhesion blocking element is prolonged by enhancing the local elastic deformability of the adhesion blocking element. In addition, the release layer adopted in the present invention is liquid, and the formed three-dimensional construct is solid, which makes the three-dimensional construct and the release layer more easily separated, thereby improving the speed of three-dimensional manufacturing.