A display substrate includes a first base and a plurality of pixel units arranged in rows and in columns. A pixel unit includes a first light-emitting device, a second light-emitting device and a third light-emitting device. A first effective light-emitting area where the first light-emitting device is located and a second effective light-emitting area where the second light-emitting device is located are arranged at intervals along a first direction, a third effective light-emitting area where the third light-emitting device is located is spaced apart from both the first effective light-emitting area and the second effective light-emitting area arranged along a second direction. A minimum distance between the first effective light-emitting area and the second effective light-emitting area along the first direction is less than a minimum distance between the third effective light-emitting area and both the first effective light-emitting area and the second effective light-emitting area along the second direction.